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dcas9 fusion sgrna library expression vector  (Addgene inc)


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    Addgene inc dcas9 fusion sgrna library expression vector

    Dcas9 Fusion Sgrna Library Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dcas9+fusion+sgrna+library+expression+vector/M29+NacMS%2BLEF%3ENac+(Plasmid+%2317179)/pmc09606310-28-0-12
    Average 93 stars, based on 5 article reviews
    dcas9 fusion sgrna library expression vector - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "CasPlay provides a gRNA-barcoded CRISPR-based display platform for antibody repertoire profiling"

    Article Title: CasPlay provides a gRNA-barcoded CRISPR-based display platform for antibody repertoire profiling

    Journal: Cell Reports Methods

    doi: 10.1016/j.crmeth.2022.100318


    Figure Legend Snippet:

    Techniques Used: Virus, Synthesized, Recombinant, Plasmid Preparation, Expressing, Software, Microarray

    Related Articles

    Virus:

    Article Title: CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-6His, rabbit Cell Signaling D3I1O; RRID: AB_2744546 Anti-HA, rabbit Cell Signaling C29F4; RRID: AB_1549585 Anti-myc, rabbit Abcam ab9106; RRID: AB_307014 Anti-FLAG M2, mouse Millipore Sigma F1804; RRID: AB_262044 Anti-FLAG, rabbit Abcam ab205606 Goat anti-mouse IgG (H&L)-Alexa 647 Abcam ab150115; RRID: AB_2687948 Goat anti-rabbit IgG (H&L)-Alexa 488 Abcam ab150077; RRID: AB_2630356 Donkey anti-rabbit IgG (H&L)-Alexa 647 Abcam ab150075; RRID: AB_2752244 Goat anti-human IgG (H&L)-Alexa 647 ThermoFisher A-21445; RRID: AB_2535862 Bacterial and virus strains Electromax DH10B E. coli ThermoFisher 18290015 BL21 (DE3) electrocompetent E. coli Sigma-Aldrich CMC0016 T7 Express lysY Competent E. coli NEB C3010I Deposited data Raw microarray image files This paper https://doi.org/10.17632/rz8ty3g3yf.1 Microarray data processing scripts (original code) This paper https://doi.org/10.17632/3kg89nrmwk.1 Link: https://github.com/kwbarber/ PICASSO Oligonucleotides SurePrint Oligonucleotide Libraries listed in Table S2 This paper (synthesized by Agilent) G7220A Oligonucleotide microarrays as PICASSO templates This paper (synthesized by CustomArray/ Genscript) N/A Primer to doublestrand dsDNAmicroarrays: 50-AC+G+G+GT+GAGGTCGGG-30, where + denotes LNA bases This paper (synthesized by IDT) N/A Recombinant DNA pdCas9-bacteria plasmid Qi et al., 2013 Addgene #44249 pgRNA-bacteria Qi et al., 2013 Addgene #44251 dCas9-fusion+sgRNA library expression vector (see Data S1) This paper Addgene #171798 dCas9-fusion+sgRNA expression scaffold (see Data S1) This paper Addgene #171799 Software and algorithms GenePix Pro 7 Molecular Devices N/A Other GenePix 4300A microarray scanner Molecular Devices GENEPIX 4300 Detailed Protocol This paper Methods S1 ..

    Microarray:

    Article Title: CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-6His, rabbit Cell Signaling D3I1O; RRID: AB_2744546 Anti-HA, rabbit Cell Signaling C29F4; RRID: AB_1549585 Anti-myc, rabbit Abcam ab9106; RRID: AB_307014 Anti-FLAG M2, mouse Millipore Sigma F1804; RRID: AB_262044 Anti-FLAG, rabbit Abcam ab205606 Goat anti-mouse IgG (H&L)-Alexa 647 Abcam ab150115; RRID: AB_2687948 Goat anti-rabbit IgG (H&L)-Alexa 488 Abcam ab150077; RRID: AB_2630356 Donkey anti-rabbit IgG (H&L)-Alexa 647 Abcam ab150075; RRID: AB_2752244 Goat anti-human IgG (H&L)-Alexa 647 ThermoFisher A-21445; RRID: AB_2535862 Bacterial and virus strains Electromax DH10B E. coli ThermoFisher 18290015 BL21 (DE3) electrocompetent E. coli Sigma-Aldrich CMC0016 T7 Express lysY Competent E. coli NEB C3010I Deposited data Raw microarray image files This paper https://doi.org/10.17632/rz8ty3g3yf.1 Microarray data processing scripts (original code) This paper https://doi.org/10.17632/3kg89nrmwk.1 Link: https://github.com/kwbarber/ PICASSO Oligonucleotides SurePrint Oligonucleotide Libraries listed in Table S2 This paper (synthesized by Agilent) G7220A Oligonucleotide microarrays as PICASSO templates This paper (synthesized by CustomArray/ Genscript) N/A Primer to doublestrand dsDNAmicroarrays: 50-AC+G+G+GT+GAGGTCGGG-30, where + denotes LNA bases This paper (synthesized by IDT) N/A Recombinant DNA pdCas9-bacteria plasmid Qi et al., 2013 Addgene #44249 pgRNA-bacteria Qi et al., 2013 Addgene #44251 dCas9-fusion+sgRNA library expression vector (see Data S1) This paper Addgene #171798 dCas9-fusion+sgRNA expression scaffold (see Data S1) This paper Addgene #171799 Software and algorithms GenePix Pro 7 Molecular Devices N/A Other GenePix 4300A microarray scanner Molecular Devices GENEPIX 4300 Detailed Protocol This paper Methods S1 ..

    Synthesized:

    Article Title: CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-6His, rabbit Cell Signaling D3I1O; RRID: AB_2744546 Anti-HA, rabbit Cell Signaling C29F4; RRID: AB_1549585 Anti-myc, rabbit Abcam ab9106; RRID: AB_307014 Anti-FLAG M2, mouse Millipore Sigma F1804; RRID: AB_262044 Anti-FLAG, rabbit Abcam ab205606 Goat anti-mouse IgG (H&L)-Alexa 647 Abcam ab150115; RRID: AB_2687948 Goat anti-rabbit IgG (H&L)-Alexa 488 Abcam ab150077; RRID: AB_2630356 Donkey anti-rabbit IgG (H&L)-Alexa 647 Abcam ab150075; RRID: AB_2752244 Goat anti-human IgG (H&L)-Alexa 647 ThermoFisher A-21445; RRID: AB_2535862 Bacterial and virus strains Electromax DH10B E. coli ThermoFisher 18290015 BL21 (DE3) electrocompetent E. coli Sigma-Aldrich CMC0016 T7 Express lysY Competent E. coli NEB C3010I Deposited data Raw microarray image files This paper https://doi.org/10.17632/rz8ty3g3yf.1 Microarray data processing scripts (original code) This paper https://doi.org/10.17632/3kg89nrmwk.1 Link: https://github.com/kwbarber/ PICASSO Oligonucleotides SurePrint Oligonucleotide Libraries listed in Table S2 This paper (synthesized by Agilent) G7220A Oligonucleotide microarrays as PICASSO templates This paper (synthesized by CustomArray/ Genscript) N/A Primer to doublestrand dsDNAmicroarrays: 50-AC+G+G+GT+GAGGTCGGG-30, where + denotes LNA bases This paper (synthesized by IDT) N/A Recombinant DNA pdCas9-bacteria plasmid Qi et al., 2013 Addgene #44249 pgRNA-bacteria Qi et al., 2013 Addgene #44251 dCas9-fusion+sgRNA library expression vector (see Data S1) This paper Addgene #171798 dCas9-fusion+sgRNA expression scaffold (see Data S1) This paper Addgene #171799 Software and algorithms GenePix Pro 7 Molecular Devices N/A Other GenePix 4300A microarray scanner Molecular Devices GENEPIX 4300 Detailed Protocol This paper Methods S1 ..

    Recombinant:

    Article Title: CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-6His, rabbit Cell Signaling D3I1O; RRID: AB_2744546 Anti-HA, rabbit Cell Signaling C29F4; RRID: AB_1549585 Anti-myc, rabbit Abcam ab9106; RRID: AB_307014 Anti-FLAG M2, mouse Millipore Sigma F1804; RRID: AB_262044 Anti-FLAG, rabbit Abcam ab205606 Goat anti-mouse IgG (H&L)-Alexa 647 Abcam ab150115; RRID: AB_2687948 Goat anti-rabbit IgG (H&L)-Alexa 488 Abcam ab150077; RRID: AB_2630356 Donkey anti-rabbit IgG (H&L)-Alexa 647 Abcam ab150075; RRID: AB_2752244 Goat anti-human IgG (H&L)-Alexa 647 ThermoFisher A-21445; RRID: AB_2535862 Bacterial and virus strains Electromax DH10B E. coli ThermoFisher 18290015 BL21 (DE3) electrocompetent E. coli Sigma-Aldrich CMC0016 T7 Express lysY Competent E. coli NEB C3010I Deposited data Raw microarray image files This paper https://doi.org/10.17632/rz8ty3g3yf.1 Microarray data processing scripts (original code) This paper https://doi.org/10.17632/3kg89nrmwk.1 Link: https://github.com/kwbarber/ PICASSO Oligonucleotides SurePrint Oligonucleotide Libraries listed in Table S2 This paper (synthesized by Agilent) G7220A Oligonucleotide microarrays as PICASSO templates This paper (synthesized by CustomArray/ Genscript) N/A Primer to doublestrand dsDNAmicroarrays: 50-AC+G+G+GT+GAGGTCGGG-30, where + denotes LNA bases This paper (synthesized by IDT) N/A Recombinant DNA pdCas9-bacteria plasmid Qi et al., 2013 Addgene #44249 pgRNA-bacteria Qi et al., 2013 Addgene #44251 dCas9-fusion+sgRNA library expression vector (see Data S1) This paper Addgene #171798 dCas9-fusion+sgRNA expression scaffold (see Data S1) This paper Addgene #171799 Software and algorithms GenePix Pro 7 Molecular Devices N/A Other GenePix 4300A microarray scanner Molecular Devices GENEPIX 4300 Detailed Protocol This paper Methods S1 ..

    Plasmid Preparation:

    Article Title: CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-6His, rabbit Cell Signaling D3I1O; RRID: AB_2744546 Anti-HA, rabbit Cell Signaling C29F4; RRID: AB_1549585 Anti-myc, rabbit Abcam ab9106; RRID: AB_307014 Anti-FLAG M2, mouse Millipore Sigma F1804; RRID: AB_262044 Anti-FLAG, rabbit Abcam ab205606 Goat anti-mouse IgG (H&L)-Alexa 647 Abcam ab150115; RRID: AB_2687948 Goat anti-rabbit IgG (H&L)-Alexa 488 Abcam ab150077; RRID: AB_2630356 Donkey anti-rabbit IgG (H&L)-Alexa 647 Abcam ab150075; RRID: AB_2752244 Goat anti-human IgG (H&L)-Alexa 647 ThermoFisher A-21445; RRID: AB_2535862 Bacterial and virus strains Electromax DH10B E. coli ThermoFisher 18290015 BL21 (DE3) electrocompetent E. coli Sigma-Aldrich CMC0016 T7 Express lysY Competent E. coli NEB C3010I Deposited data Raw microarray image files This paper https://doi.org/10.17632/rz8ty3g3yf.1 Microarray data processing scripts (original code) This paper https://doi.org/10.17632/3kg89nrmwk.1 Link: https://github.com/kwbarber/ PICASSO Oligonucleotides SurePrint Oligonucleotide Libraries listed in Table S2 This paper (synthesized by Agilent) G7220A Oligonucleotide microarrays as PICASSO templates This paper (synthesized by CustomArray/ Genscript) N/A Primer to doublestrand dsDNAmicroarrays: 50-AC+G+G+GT+GAGGTCGGG-30, where + denotes LNA bases This paper (synthesized by IDT) N/A Recombinant DNA pdCas9-bacteria plasmid Qi et al., 2013 Addgene #44249 pgRNA-bacteria Qi et al., 2013 Addgene #44251 dCas9-fusion+sgRNA library expression vector (see Data S1) This paper Addgene #171798 dCas9-fusion+sgRNA expression scaffold (see Data S1) This paper Addgene #171799 Software and algorithms GenePix Pro 7 Molecular Devices N/A Other GenePix 4300A microarray scanner Molecular Devices GENEPIX 4300 Detailed Protocol This paper Methods S1 ..

    Article Title: CasPlay provides a gRNA-barcoded CRISPR-based display platform for antibody repertoire profiling
    Article Snippet: pdCas9-bacteria plasmid , , Addgene #44249. .. dCas9-fusion + sgRNA library expression vector (see Supplementary File 2) , , Addgene #171798. .. dCas9-fusion + sgRNA expression scaffold (see Supplementary File 2) , , Addgene #171799.

    Expressing:

    Article Title: CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-6His, rabbit Cell Signaling D3I1O; RRID: AB_2744546 Anti-HA, rabbit Cell Signaling C29F4; RRID: AB_1549585 Anti-myc, rabbit Abcam ab9106; RRID: AB_307014 Anti-FLAG M2, mouse Millipore Sigma F1804; RRID: AB_262044 Anti-FLAG, rabbit Abcam ab205606 Goat anti-mouse IgG (H&L)-Alexa 647 Abcam ab150115; RRID: AB_2687948 Goat anti-rabbit IgG (H&L)-Alexa 488 Abcam ab150077; RRID: AB_2630356 Donkey anti-rabbit IgG (H&L)-Alexa 647 Abcam ab150075; RRID: AB_2752244 Goat anti-human IgG (H&L)-Alexa 647 ThermoFisher A-21445; RRID: AB_2535862 Bacterial and virus strains Electromax DH10B E. coli ThermoFisher 18290015 BL21 (DE3) electrocompetent E. coli Sigma-Aldrich CMC0016 T7 Express lysY Competent E. coli NEB C3010I Deposited data Raw microarray image files This paper https://doi.org/10.17632/rz8ty3g3yf.1 Microarray data processing scripts (original code) This paper https://doi.org/10.17632/3kg89nrmwk.1 Link: https://github.com/kwbarber/ PICASSO Oligonucleotides SurePrint Oligonucleotide Libraries listed in Table S2 This paper (synthesized by Agilent) G7220A Oligonucleotide microarrays as PICASSO templates This paper (synthesized by CustomArray/ Genscript) N/A Primer to doublestrand dsDNAmicroarrays: 50-AC+G+G+GT+GAGGTCGGG-30, where + denotes LNA bases This paper (synthesized by IDT) N/A Recombinant DNA pdCas9-bacteria plasmid Qi et al., 2013 Addgene #44249 pgRNA-bacteria Qi et al., 2013 Addgene #44251 dCas9-fusion+sgRNA library expression vector (see Data S1) This paper Addgene #171798 dCas9-fusion+sgRNA expression scaffold (see Data S1) This paper Addgene #171799 Software and algorithms GenePix Pro 7 Molecular Devices N/A Other GenePix 4300A microarray scanner Molecular Devices GENEPIX 4300 Detailed Protocol This paper Methods S1 ..

    Article Title: CasPlay provides a gRNA-barcoded CRISPR-based display platform for antibody repertoire profiling
    Article Snippet: pdCas9-bacteria plasmid , , Addgene #44249. .. dCas9-fusion + sgRNA library expression vector (see Supplementary File 2) , , Addgene #171798. .. dCas9-fusion + sgRNA expression scaffold (see Supplementary File 2) , , Addgene #171799.

    Software:

    Article Title: CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-6His, rabbit Cell Signaling D3I1O; RRID: AB_2744546 Anti-HA, rabbit Cell Signaling C29F4; RRID: AB_1549585 Anti-myc, rabbit Abcam ab9106; RRID: AB_307014 Anti-FLAG M2, mouse Millipore Sigma F1804; RRID: AB_262044 Anti-FLAG, rabbit Abcam ab205606 Goat anti-mouse IgG (H&L)-Alexa 647 Abcam ab150115; RRID: AB_2687948 Goat anti-rabbit IgG (H&L)-Alexa 488 Abcam ab150077; RRID: AB_2630356 Donkey anti-rabbit IgG (H&L)-Alexa 647 Abcam ab150075; RRID: AB_2752244 Goat anti-human IgG (H&L)-Alexa 647 ThermoFisher A-21445; RRID: AB_2535862 Bacterial and virus strains Electromax DH10B E. coli ThermoFisher 18290015 BL21 (DE3) electrocompetent E. coli Sigma-Aldrich CMC0016 T7 Express lysY Competent E. coli NEB C3010I Deposited data Raw microarray image files This paper https://doi.org/10.17632/rz8ty3g3yf.1 Microarray data processing scripts (original code) This paper https://doi.org/10.17632/3kg89nrmwk.1 Link: https://github.com/kwbarber/ PICASSO Oligonucleotides SurePrint Oligonucleotide Libraries listed in Table S2 This paper (synthesized by Agilent) G7220A Oligonucleotide microarrays as PICASSO templates This paper (synthesized by CustomArray/ Genscript) N/A Primer to doublestrand dsDNAmicroarrays: 50-AC+G+G+GT+GAGGTCGGG-30, where + denotes LNA bases This paper (synthesized by IDT) N/A Recombinant DNA pdCas9-bacteria plasmid Qi et al., 2013 Addgene #44249 pgRNA-bacteria Qi et al., 2013 Addgene #44251 dCas9-fusion+sgRNA library expression vector (see Data S1) This paper Addgene #171798 dCas9-fusion+sgRNA expression scaffold (see Data S1) This paper Addgene #171799 Software and algorithms GenePix Pro 7 Molecular Devices N/A Other GenePix 4300A microarray scanner Molecular Devices GENEPIX 4300 Detailed Protocol This paper Methods S1 ..



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    dcas9 fusion sgrna library expression vector  (Addgene inc)
    93
    Addgene inc dcas9 fusion sgrna library expression vector

    Dcas9 Fusion Sgrna Library Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dcas9+fusion+sgrna+library+expression+vector/M29+NacMS%2BLEF%3ENac+(Plasmid+%2317179)/pmc09606310-28-0-12
    Average 93 stars, based on 1 article reviews
    dcas9 fusion sgrna library expression vector - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Journal: Cell Reports Methods

    Article Title: CasPlay provides a gRNA-barcoded CRISPR-based display platform for antibody repertoire profiling

    doi: 10.1016/j.crmeth.2022.100318

    Figure Lengend Snippet:

    Article Snippet: dCas9-fusion + sgRNA library expression vector (see Supplementary File 2) , , Addgene #171798.

    Techniques: Virus, Synthesized, Recombinant, Plasmid Preparation, Expressing, Software, Microarray